CLIA Kit for Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

  • CLIA Kit for Leptin (LEP) Packages (Simulation)
  • CLIA Kit for Leptin (LEP) Packages (Simulation)
  • CLIA Kit for Leptin (LEP) Results demonstration
  • SCA084Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Leptin (LEP)

This assay has high sensitivity and excellent specificity for detection of Leptin (LEP).
No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.

Recovery of the CLIA Kit for Leptin (LEP)

Matrices listed below were spiked with certain level of recombinant Leptin (LEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Leptin (LEP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 88-96 92
heparin plasma(n=5) 80-101 97

Precision of the CLIA Kit for Leptin (LEP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Leptin (LEP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leptin (LEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-103% 84-104% 93-103% 85-99%
EDTA plasma(n=5) 95-104% 98-105% 97-105% 84-98%
heparin plasma(n=5) 82-101% 79-101% 83-91% 87-95%

Stability of the CLIA Kit for Leptin (LEP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Leptin (LEP)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Leptin (LEP)

The microplate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Leptin (LEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Leptin (LEP) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APA084Hu01 Active Leptin (LEP) Cell culture; Activity Assays.
RPA084Hu01 Recombinant Leptin (LEP) Positive Control; Immunogen; SDS-PAGE; WB.
PAA084Hu01 Polyclonal Antibody to Leptin (LEP) WB; IHC; ICC; IP.
LAA084Hu71 Biotin-Linked Polyclonal Antibody to Leptin (LEP) WB; IHC; ICC.
LAA084Hu81 FITC-Linked Polyclonal Antibody to Leptin (LEP) WB; IHC; ICC; IF.
MAA084Hu22 Monoclonal Antibody to Leptin (LEP) WB; IHC; ICC; IP.
MAA084Hu21 Monoclonal Antibody to Leptin (LEP) WB; IHC; ICC; IP.
HEA084Hu High Sensitive ELISA Kit for Leptin (LEP) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA084Hu ELISA Kit for Leptin (LEP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA084Hu CLIA Kit for Leptin (LEP) Chemiluminescent immunoassay for Antigen Detection.
KSA084Hu01 ELISA Kit DIY Materials for Leptin (LEP) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK