Active Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
- Product No.APA084Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point5.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors!US$ 300
For more details, please contact local distributors! US$ 600
For more details, please contact local distributors! US$ 1800
For more details, please contact local distributors! US$ 4500
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant LEP, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Leptin (LEP)
Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Many of leptin's effects are mediated through neuropeptide- containing neurons and neuropeptide receptors in the hypothalamus. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Besides, Hexokinase 3, White Cell (HK3) has been identified as an interactor of LEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LEP and recombinant human HK3. Briefly, LEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HK3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LEPpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of LEP and HK3 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Leptin (LEP)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Leptin (LEP)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Leptin (LEP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APA084Hu01||Active Leptin (LEP)||Cell culture; Activity Assays.|
|RPA084Hu01||Recombinant Leptin (LEP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA084Hu01||Polyclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IP.|
|LAA084Hu71||Biotin-Linked Polyclonal Antibody to Leptin (LEP)||WB; IHC; ICC.|
|LAA084Hu81||FITC-Linked Polyclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IF.|
|MAA084Hu22||Monoclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IP.|
|MAA084Hu21||Monoclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IP.|
|HEA084Hu||High Sensitive ELISA Kit for Leptin (LEP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEA084Hu||ELISA Kit for Leptin (LEP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA084Hu||CLIA Kit for Leptin (LEP)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA084Hu01||ELISA Kit DIY Materials for Leptin (LEP)||Main materials for "Do It (ELISA Kit) Yourself".|