Polyclonal Antibody to Heparanase (HPA)

HPSE; HPR1; HPSE1; HSE1; Endo-glucoronidase;

  • Polyclonal Antibody to Heparanase (HPA) Packages (Simulation)
  • Polyclonal Antibody to Heparanase (HPA) Packages (Simulation)
  • Polyclonal Antibody to Heparanase (HPA) Used in DAB staining on fromalin fixed paraffin-embedded Spleen tissue
  • PAA711Hu02.jpg Figure. Western Blot; Sample: Recombinant HPA, Human.
  • Polyclonal Antibody to Heparanase (HPA) Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human HepG2 Cells; Lane3: Human Liver Tissue.
  • Polyclonal Antibody to Heparanase (HPA) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: HPA knockout HepG2 cell lysate;
    Predicted MW: 43,53,55,61kd
    Observed MW: 68kd
    Primary Ab: 1µg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparanase (HPA) HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HPA(HPSE) Polyclonal Antibody (Catalog # PAA711Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Heparanase (HPA)

The antibody is a rabbit polyclonal antibody raised against HPA. It has been selected for its ability to recognize HPA in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Heparanase (HPA)

Western blotting: 0.5-2µg/mL;1:310-1200
Immunohistochemistry: 5-20µg/mL;1:31-120
Immunocytochemistry: 5-20µg/mL;1:31-120
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Heparanase (HPA)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Heparanase (HPA)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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