Polyclonal Antibody to Heparanase (HPSE)

HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;

  • Polyclonal Antibody to Heparanase (HPSE) Packages (Simulation)
  • Polyclonal Antibody to Heparanase (HPSE) Packages (Simulation)
  • Polyclonal Antibody to Heparanase (HPSE) DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked
  • Polyclonal Antibody to Heparanase (HPSE) DAB staining on IHC-P;
    Sample: Human Colon Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparanase (HPSE) DAB staining on IHC-P;
    Sample: Human Spleen Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparanase (HPSE) FITC staining on IF;
    Samples: Human HepG2 cell;
    Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Heparanase (HPSE) FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human HPSE Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA711Hu02.jpg Figure. Western Blot; Sample: Recombinant HPA, Human.
  • Polyclonal Antibody to Heparanase (HPSE) Western Blot; Samples: Lane1: LO2 cell lysate; Lane2: HepG2 cell lysate; Lane3: Jurkat cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human HPSE Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparanase (HPSE) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: HPA knockout HepG2 cell lysate;
    Predicted MW: 43,53,55,61kd
    Observed MW: 68kd
    Primary Ab: 1µg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Heparanase (HPSE)

The antibody is a rabbit polyclonal antibody raised against HPSE. It has been selected for its ability to recognize HPSE in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Heparanase (HPSE)

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Heparanase (HPSE)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Heparanase (HPSE)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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