Active Heparanase (HPSE)

HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;

  • Active Heparanase (HPSE) Packages (Simulation)
  • Active Heparanase (HPSE) Packages (Simulation)
  • APA711Hu04.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Heparanase (HPSE)

Heparanase (HPSE) selectively cleaves heparan sulfate at specific sites on heparan sulfate proteoglycans (HSPGs). HPSE facilitates cell migration associated with metastasis, wound healing and inflammation. An increase in its activity is associated with an increase in VEGF activity, which further enhances angiogenesis. HPSE also enhances shedding of syndecans and increases endothelial invasion and angiogenesis in myelomas. It acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. In addition, it increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. HPSE is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. The enzyme activity of recombinant human HPSE was assayed in an ELISA format using non-reducing end biotinylated heparan sulfate on recombinant human syndecan 4 as a substrate. Briefly, combining 10 µL of different concentrations of rhHPSE with 10 µL of the biotinylated-syndecan 4 in a vial (10 µL of assay buffer and 10 µL of biotinylated-syndecan 4 as a negative control) and incubated for 2h at 37℃. After incubation, 220 µL of PBS, with 1% BSA (pH7.4) was added to each reaction, then loading 100 ul of each sample onto the anti-syndecan 4 pAb-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. As a result, 500 ng of recombinant human HPSE digestion will result in >50% of OD reduction compared with the negative control.

USAGE of the Active Heparanase (HPSE)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Heparanase (HPSE)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Heparanase (HPSE)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA711Hu04 Recombinant Heparanase (HPSE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu02 Recombinant Heparanase (HPSE) Positive Control; Immunogen; SDS-PAGE; WB.
APA711Hu04 Active Heparanase (HPSE) Cell culture; Activity Assays.
APA711Hu01 Active Heparanase (HPSE) Cell culture; Activity Assays.
EPA711Hu61 Eukaryotic Heparanase (HPSE) Positive Control; Immunogen; SDS-PAGE; WB.
APA711Hu61 Active Heparanase (HPSE) Cell culture; Activity Assays.
RPA711Hu01 Recombinant Heparanase (HPSE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu03 Recombinant Heparanase (HPSE) Positive Control; Immunogen; SDS-PAGE; WB.
CPA711Hu21 OVA Conjugated Heparanase (HPSE) Immunogen; SDS-PAGE; WB.
PAA711Hu04 Polyclonal Antibody to Heparanase (HPSE) WB; IHC; ICC; IP.
PAA711Hu02 Polyclonal Antibody to Heparanase (HPSE) WB,IHC,ICC/IF
PAA711Hu01 Polyclonal Antibody to Heparanase (HPSE) WB; IHC; ICC; IP.
PAA711Hu03 Polyclonal Antibody to Heparanase (HPSE) WB; IHC; ICC; IP.
FAA711Hu01 Anti-Heparanase (HPSE) Polyclonal Antibody Flow cytometry.
LAA711Hu71 Biotin-Linked Polyclonal Antibody to Heparanase (HPSE) WB; IHC; ICC.
MAA711Hu23 Monoclonal Antibody to Heparanase (HPSE) WB
MAA711Hu22 Monoclonal Antibody to Heparanase (HPSE) WB; IHC; ICC; IP.
MAA711Hu21 Monoclonal Antibody to Heparanase (HPSE) WB,ICC/IF
SEA711Hu ELISA Kit for Heparanase (HPSE) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA711Hu High Sensitive ELISA Kit for Heparanase (HPSE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA711Hu Multiplex Assay Kit for Heparanase (HPSE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA711Hu01 ELISA Kit DIY Materials for Heparanase (HPSE) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK