Active Angiotensin I Converting Enzyme 2 (ACE2)
ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15
- Product No.APB886Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 280
For more details, please contact local distributors!US$ 700
For more details, please contact local distributors! US$ 1400
For more details, please contact local distributors! US$ 4200
For more details, please contact local distributors! US$ 10500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Angiotensin I Converting Enzyme 2 (ACE2)
Angiotensin I Converting Enzyme 2 (ACE2), as a transmembrane protein, serves as the main entry point into cells for some coronaviruses. More specifically, the binding of the spike S1 protein of SARS-CoV and SARS-CoV-2 to the enzymatic domain of ACE2 on the surface of cells results in endocytosis and translocation of both the virusand the enzyme into endosomes located within cells. The activity of recombinant rat ACE2 is measured by its ability to cleave a fluorogenic peptide substrate MCA-Tyr-Val-Ala-Asp-Ala-Pro-Lys(DNP)-OH in the assay buffer 50 mM Tris, 1 M NaCl, pH 7.5. The rrACE2 is diluted to 0.5 ug/mL in assay buffer. Loading into a black well plate 50 µL of 0.5 ug/mL rrACE2 and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rrACE2. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant rat ACE2 is > 2200 pmol/min/µg.
USAGE of the Active Angiotensin I Converting Enzyme 2 (ACE2)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Angiotensin I Converting Enzyme 2 (ACE2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Angiotensin I Converting Enzyme 2 (ACE2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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