Recombinant Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1
- Product No.URAA788Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceRecombinant monoclonal antibody preparation
- HostMouse
- Potency (Clone Number)n/a
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IF; ICC; IHC; IP; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download n/a
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors! US$ 2875
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Packages (Simulation)
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Packages (Simulation)
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AF488 staining on IF;
Sample: MDA-MB-231 cell
Primary Ab: 10µg/ml Recombinant Mouse Anti-Human PDCD1LG1 Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Human SK-N-SH line was stained with 0.01µg/ml mouse Anti-human PDCD1LG1 Recombinant Antibody (Catalog RAA788Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Human MDA-MB-231 line was stained with 0.01µg/ml mouse Anti-human PDCD1LG1 Recombinant Antibody (Catalog RAA788Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Recombinant Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
The antibody is a mouse monoclonal antibody raised against PDL1. It has been selected for its ability to recognize PDL1 in immunohistochemical staining and western blotting.
USAGE of the Recombinant Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:0.01μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Recombinant Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Recombinant Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Mouse Antibody Variable Region Gene Amplification Kit
Real Time PCR Experimental Service