Polyclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1
- Product No.UPAA788Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA788Hu01-Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDL1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 47
For more details, please contact local distributors!US$ 111
For more details, please contact local distributors! US$ 158
For more details, please contact local distributors! US$ 395
For more details, please contact local distributors! US$ 1580
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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FITC staining on IF; Sample: Human Hela cell; Primary Ab: 20ug/ml Rabbit Anti-Human PDCD1LG1 Antibody Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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AF488 staining on IF;
Sample: 293F cell
Primary Ab: 30µg/ml Rabbit Anti-Human PDCD1LG1 Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Rat Heart lysate; Lane3: Rat Skeletal muscle lysate Primary Ab: 0.1µg/ml Rabbit Anti-Human PDCD1LG1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Mouse Heart lysate
Primary Ab: 2µg/ml Rabbit Anti-Human PDCD1LG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Knockout Varification:
Lane 1: Wild-type MDA-MB-231 cell lysate;
Lane 2: PDCD1LG1 knockout MB231 cell lysate;
Predicted MW: 33,20kDa
Observed MW: 33kDa
Primary Ab: 1µg/ml Rabbit Anti-Human PDCD1LG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Mouse Heart lysate; Lane3: Porcine Heart lysate
Primary Ab: 0.5µg/ml Rabbit Anti-Human PDCD1LG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: Porcine Skeletal muscle lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human PDCD1LG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: 293F cell lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human PDCD1LG1 Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
The antibody is a rabbit polyclonal antibody raised against PDL1. It has been selected for its ability to recognize PDL1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Western blotting: 0.01-3µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service