Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
- Product No.UMAE038Hu23
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)E5
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPE038Hu02-Recombinant Neurofilament, Light Polypeptide (NEFL)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.92mg/mL
- Organism Species MoreCavia (Guinea pig ), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Rabbit Cerebrum lysate; Lane3: Cavia Cerebrum lysate Primary Ab: 1µg/ml Mouse Anti-Human NEFL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
The antibody is a mouse monoclonal antibody raised against NEFL. It has been selected for its ability to recognize NEFL in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service