ELISA Kit DIY Materials for Visfatin (VF)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
- Product No.KSA638Hu11
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ng/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Download Instruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Picture
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Visfatin (VF)
The Abs in the kit have high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Visfatin (VF)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Visfatin (VF)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Visfatin (VF)
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| KSA638Hu11 | ELISA Kit DIY Materials for Visfatin (VF) | Main materials for "Do It (ELISA Kit) Yourself". |