Polyclonal Antibody to Visfatin (VF)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
- Product No.UPAA638Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA638Hu01-Recombinant Visfatin (VF)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 47
For more details, please contact local distributors!US$ 111
For more details, please contact local distributors! US$ 158
For more details, please contact local distributors! US$ 395
For more details, please contact local distributors! US$ 1580
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Esophagus Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human VF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human VF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human VF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human VF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant VF, Human.
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Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human VF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human VF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: Porcine Stomach lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human VF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: 293T cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human VF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: VF knockout 293T cell lysate;
Predicted MW: 55kd
Observed MW: 55kd
Primary Ab: 1µg/ml Rabbit Anti-Human VF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Visfatin (VF)
The antibody is a rabbit polyclonal antibody raised against VF. It has been selected for its ability to recognize VF in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Visfatin (VF)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Visfatin (VF)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Visfatin (VF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
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