Active Resistin (RETN)

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

  • Active Resistin (RETN) Packages (Simulation)
  • Active Resistin (RETN) Packages (Simulation)
  • APA847Hu61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Resistin (RETN)

Resistin (resistance-to-insulin, RETN) is a 10 kDa member of a small family of secreted cysteine-rich peptide hormones which also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3). Human Resistin precursor is 108 amino acids (aa) in length. It contains an 18 aa signal sequence plus a 90 aa mature region. These molecules purportedly play some role in inflammation, glucose metabolism, and angiogenesis. Besides, Toll Like Receptor 4 (TLR4) has been identified as an interactor of RETN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RETN and recombinant human TLR4. Briefly, RETN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TLR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RETN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of RETN and TLR4 was shown in Figure 1, the ED50 for this effect is 5.13 ug/mL.

USAGE of the Active Resistin (RETN)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Resistin (RETN)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Resistin (RETN)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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