Active Phospholipase A2 Group VII (LpPLA2)
PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated
- Product No.APA867Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point7.1
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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WB Image
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Phospholipase A2 Group VII (LpPLA2)
Phospholipase A2 Group VII (PLA2G7) which is also known as Lp-PLA2, is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL. It is produced in major by mature macrophages and activated platelets and catalyzes the degradation of platelet-activating factor to biologically inactive products. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PLA2G7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PLA2G7 and recombinant rat PON1. Briefly, PLA2G7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PON1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PLA2G7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PLA2G7 and recombinant rat PON1 was shown in Figure 1, the EC50 for this effect is 0.7 ug/mL.
USAGE of the Active Phospholipase A2 Group VII (LpPLA2)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Phospholipase A2 Group VII (LpPLA2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Phospholipase A2 Group VII (LpPLA2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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