Mouse Model for Tumor Transplantation (TT)
- Product No.DSI530Mu05
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Prototype SpeciesHuman
- SourceInduced by A549-luc intravenous transplantation
- Model Animal StrainsBalb/c-nude Mice(SPF), healthy, male, age: 4~5weeks, body weight:18g~20g.
- Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
- Modeling Period4-6 weeks
- Modeling MethodCell line: human lung cancer cell line A549-LUC marked with luciferase gene
Tumor formation by tail vein injection in nude mice: Cells with good growth were digested and prepared into cell suspension. After counting, 100μ L (containing 2*106 cells) of cell suspension was taken and inoculated into nude mice through tail vein. Normal diet was carried out every day.
28 days after injection, in vivo imaging was performed.
Procedures for imaging small animals in vivo:
Fluorescence substrate injection: Sodium fluorescein D was injected intraperitoneally, and 15 mg/mL fluorescein working solution was added at the concentration of 10 uL/g. Injection after 10 minutes, began to fluorescence in vivo imaging.
Open the imaging system of small animals in vivo and set the instrument parameters to select a wavelength range of 500-620nm according to the wavelength of the target protein 530-600nm. Select Fluorescent mode, put the mouse into the imaging camera platform, the software controls the platform to rise and fall to a suitable field of view, automatically turn on the light (bright field) to take the first background image. Next, the light was turned off automatically and the specific photons emitted by the mice were photographed in the dark field without external light. Superposition of bright field and dark field background can intuitively display the location and intensity of specific photons in the animal body to complete the imaging operation. It should be noted that appropriate excitation and emission filters should be selected for fluorescence imaging, while bioluminescence needs to be stimulated by substrate injection before imaging.
- ApplicationsDisease Model
- Download n/a
- UOM Each case
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Fig. Fluorescence in vivo imaging after A549-luc cells were injected in situ for 4w
- Fig. The lung of nude mice injected by A549
- ISO9001: 2008, ISO13485: 2003 Registered
Model Evaluation of the Mouse Model for Tumor Transplantation (TT)
28d live imaging, the lungs were imaged visibly;
Pathological Results of the Mouse Model for Tumor Transplantation (TT)
Cytokines Level of the Mouse Model for Tumor Transplantation (TT)
Statistical Analysis of the Mouse Model for Tumor Transplantation (TT)
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
Tissue/Sections Customized Service
Serums Customized Service
Immunohistochemistry (IHC) Experiment Service
Small Animal In Vivo Imaging Experiment Service
Small Animal Micro CT Imaging Experiment Service
Small Animal MRI Imaging Experiment Service
Small Animal Ultrasound Imaging Experiment Service
Transmission Electron Microscopy (TEM) Experiment Service
Scanning Electron Microscope (SEM) Experiment Service
Learning and Memory Behavioral Experiment Service
Anxiety and Depression Behavioral Experiment Service
Drug Addiction Behavioral Experiment Service
Pain Behavioral Experiment Service
Neuropsychiatric Disorder Behavioral Experiment Service
Fatigue Behavioral Experiment Service
Nitric Oxide Assay Kit (A012)
Nitric Oxide Assay Kit (A013-2)
Total Anti-Oxidative Capability Assay Kit（A015-2）
Total Anti-Oxidative Capability Assay Kit (A015-1)
Superoxide Dismutase Assay Kit
Fructose Assay Kit (A085)
Citric Acid Assay Kit (A128 )
Catalase Assay Kit
Malondialdehyde Assay Kit
Glutathione S-Transferase Assay Kit
Microscale Reduced Glutathione assay kit
Glutathione Reductase Activity Coefficient Assay Kit
Angiotensin Converting Enzyme Kit
Glutathione Peroxidase (GSH-PX) Assay Kit
Cloud-Clone Multiplex assay kits
|Catalog No.||Organism species: Mus musculus (Mouse)||Applications (RESEARCH USE ONLY!)|
|DSI530Mu01||Mouse Model for Tumor Transplantation (TT)||TE-1 transplanted tumor cells model|
|DSI530Mu02||Mouse Model for Tumor Transplantation (TT)||Disease Model for HepG2-luc in situ tumor formation|
|DSI530Mu03||Mouse Model for Tumor Transplantation (TT)||Tumor metastasis disease model|
|DSI530Mu04||Mouse Model for Tumor Transplantation (TT)||Disease Model|
|DSI530Mu05||Mouse Model for Tumor Transplantation (TT)||Disease Model|
|DSI530Mu06||Mouse Model for Tumor Transplantation (TT)||n/a|
|DSI530Mu07||Mouse Model for Tumor Transplantation (TT)||n/a|
|DSI530Mu08||Mouse Model for Tumor Transplantation (TT)||n/a|