Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- Product No.MAA591Mi21
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C4
- Ig Isotype IgG2b Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA591Mi01-Recombinant Proliferating Cell Nuclear Antigen (PCNA)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreCavia (Guinea pig ), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 125
For more details, please contact local distributors!US$ 293
For more details, please contact local distributors! US$ 418
For more details, please contact local distributors! US$ 1045
For more details, please contact local distributors! US$ 4180
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 20ug/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20ug/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 20ug/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Mammary gland Tissue;
Primary Ab: 20ug/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 20ug/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Mammary gland Tissue
Primary Ab: 20µg/ml Mouse Anti-Multi-species PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 20µg/ml Mouse Anti-Multi-species PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Mouse Anti-Multi-species PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human MCF7 cell;
Primary Ab: 20μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.75μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
Sample: MCF7 cell
Primary Ab: 20µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: 293T cell lysate Primary Ab: 0.01µg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: ECV304 cell lysate; Lane3: Mouse Testis lysate; Lane4: Mouse Small Intestine lysate; Lane5: Mouse Thymus lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Porcine Spleen lysate; Lane3: Bovine Heart lysate; Lane4: Canine Liver lysate; Lane5: Cavia Spleen lysate; Lane6: Caprine Cerebrum lysate
Primary Ab: 2µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
The antibody is a mouse monoclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service