Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- Product No.MAA591Mi22
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C7
- Ig Isotype IgG2b Kappa
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA591Mi01-Recombinant Proliferating Cell Nuclear Antigen (PCNA)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreCavia (Guinea pig ), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 128
For more details, please contact local distributors!US$ 300
For more details, please contact local distributors! US$ 428
For more details, please contact local distributors! US$ 1070
For more details, please contact local distributors! US$ 4280
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 10µg/ml Mouse Anti-Multi-species PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Rabbit Pancreas lysate Primary Ab: 2µg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: K562 cell lysate; Lane7: MCF7 cell lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: U87MG cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Spleen lysate; Lane3: Mouse Testis lysate; Lane4: Mouse Small intestine lysate; Lane5: Mouse Lymph node lysate; Lane6: Mouse Ovary lysate; Lane7: Mouse Thymus lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Mouse Cerebrum lysate; Lane4: Rat Cerebrum lysate; Lane5: Rat Liver lysate; Lane6: Cavia Cerebrum lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Porcine Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Gallus Cerebrum lysate; Lane5: Equine Liver lysate; Lane6: Caprine Cerebrum lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
The antibody is a mouse monoclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service