Instant ELISA Kit for Interleukin 8 (IL8)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

  • Instant ELISA Kit for Interleukin 8 (IL8) Packages (Simulation)
  • Instant ELISA Kit for Interleukin 8 (IL8) Packages (Simulation)
  • Instant ELISA Kit for Interleukin 8 (IL8) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Instant ELISA Kit for Interleukin 8 (IL8)

This assay has high sensitivity and excellent specificity for detection of Instant Interleukin 8 (IL8).
No significant cross-reactivity or interference between Instant Interleukin 8 (IL8) and analogues was observed.

Recovery of the Instant ELISA Kit for Interleukin 8 (IL8)

Matrices listed below were spiked with certain level of recombinant Instant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Interleukin 8 (IL8) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-98 94
EDTA plasma(n=5) 95-103 101
heparin plasma(n=5) 89-101 97

Precision of the Instant ELISA Kit for Interleukin 8 (IL8)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Instant ELISA Kit for Interleukin 8 (IL8)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 98-105% 91-99% 92-101%
EDTA plasma(n=5) 80-89% 94-101% 96-104% 88-99%
heparin plasma(n=5) 96-103% 89-102% 88-96% 95-105%

Stability of the Instant ELISA Kit for Interleukin 8 (IL8)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Instant ELISA Kit for Interleukin 8 (IL8)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the Instant ELISA Kit for Interleukin 8 (IL8)

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interleukin 8 (IL8). Standards or samples and HRP-labeled detection antibody specific to Instant Interleukin 8 (IL8) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interleukin 8 (IL8), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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