CLIA Kit for Myeloperoxidase (MPO)

  • CLIA Kit for Myeloperoxidase (MPO) Packages (Simulation)
  • CLIA Kit for Myeloperoxidase (MPO) Packages (Simulation)
  • CLIA Kit for Myeloperoxidase (MPO) Results demonstration
  • SCA601Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Myeloperoxidase (MPO)

This assay has high sensitivity and excellent specificity for detection of Myeloperoxidase (MPO).
No significant cross-reactivity or interference between Myeloperoxidase (MPO) and analogues was observed.

Recovery of the CLIA Kit for Myeloperoxidase (MPO)

Matrices listed below were spiked with certain level of recombinant Myeloperoxidase (MPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Myeloperoxidase (MPO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 83-97 94
heparin plasma(n=5) 81-92 87

Precision of the CLIA Kit for Myeloperoxidase (MPO)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Myeloperoxidase (MPO)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myeloperoxidase (MPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 81-94% 94-102% 78-93%
EDTA plasma(n=5) 95-102% 80-102% 88-102% 81-105%
heparin plasma(n=5) 98-105% 85-93% 79-97% 83-98%

Stability of the CLIA Kit for Myeloperoxidase (MPO)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Myeloperoxidase (MPO)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Myeloperoxidase (MPO)

The microplate provided in this kit has been pre-coated with an antibody specific to Myeloperoxidase (MPO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myeloperoxidase (MPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myeloperoxidase (MPO) level in the sample or standard.;

Related products

Catalog No. Organism species: Rattus norvegicus (Rat) Applications (RESEARCH USE ONLY!)
RPA601Ra01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
RPA601Ra02 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
PAA601Ra01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
MAA601Ra21 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
SEA601Ra ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA601Ra CLIA Kit for Myeloperoxidase (MPO) Chemiluminescent immunoassay for Antigen Detection.
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