Polyclonal Antibody to Tumor Protein p53 (P53)

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • Polyclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (P53) FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Tumor Protein p53 (P53) DAB staining on IHC-P;
    Sample: Porcine Colon Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human P53 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) DAB staining on IHC-P;
    Sample: Porcine Lymph node Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human P53 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA928Hu01.jpg Figure 1. Western Blot; Sample: Recombinant TP53, Human.
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Human 293T cell lysate
    Primary Ab: 1µg/mL Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: TP53 knockout MCF7 cell lysate;
    Predicted MW: 24,30,33,34,38,39,44kDa
    Observed MW: 50kDa
    Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Porcine Skin lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Tumor Protein p53 (P53)

The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Tumor Protein p53 (P53)

Western blotting: 0.01-2µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Tumor Protein p53 (P53)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Tumor Protein p53 (P53)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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