Active Tumor Protein p53 (P53)
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- Product No.APA928Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point9.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors!US$ 468
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Gene Sequencing (Extract)
- Figure. SDS-PAGE
- Figure. Western Blot
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Tumor Protein p53 (P53)
Figure. Inhibition of Jurkat cell proliferation after stimulated with TP53
Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation- related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). TP53 involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. To test the effect of TP53 on cell apoptosis, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human TP53. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of Jurkat cells after incubation with TP53 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TP53 for 72h. The result was shown in Figure 2. It was obvious that TP53 significantly inhibit cell viability of Jurkat cells.
(A) Jurkat cells cultured in 1640, stimulated with 1ug/mL TP53 for 72h;
(B) Unstimulated Jurkat cells cultured in 1640 for 72h.
Figure. Inhibition of Jurkat cell proliferation after stimulated with TP53.
USAGE of the Active Tumor Protein p53 (P53)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Tumor Protein p53 (P53)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Tumor Protein p53 (P53)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPA928Hu01||Recombinant Tumor Protein p53 (P53)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA928Hu02||Recombinant Tumor Protein p53 (P53)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA928Hu03||Recombinant Tumor Protein p53 (P53)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA928Hu01||Active Tumor Protein p53 (P53)||Cell culture; Activity Assays.|
|PAA928Hu01||Polyclonal Antibody to Tumor Protein p53 (P53)||WB; IHC; ICC; IP.|
|LAA928Hu71||Biotin-Linked Polyclonal Antibody to Tumor Protein p53 (P53)||WB; IHC; ICC.|
|MAA928Hu22||Monoclonal Antibody to Tumor Protein p53 (P53)||WB; IHC; ICC; IP.|
|SEA928Hu||ELISA Kit for Tumor Protein p53 (P53)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA928Hu||CLIA Kit for Tumor Protein p53 (P53)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA928Hu01||ELISA Kit DIY Materials for Tumor Protein p53 (P53)||Main materials for "Do It (ELISA Kit) Yourself".|