Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Used in DAB staining on fromalin fixed paraffin-embedded Brain tissue
  • PAA693Hu01.jpg WB Image
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Human Lung lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: HSPB1 knockout Hela cell lysate;
    Predicted MW: 23kd
    Observed MW: 23kd
    Primary Ab: 3µg/ml Rabbit Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Porcine Skeletal muscle lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Porcine Heart lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

The antibody is a rabbit polyclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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