Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HSPB27 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P;
    Samples: Human Cardiac Muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P;
    Samples: Human Skeletal muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P;
    Samples: Human Placenta Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA693Hu01.jpg Figure. Western Blot; Sample: Recombinant Hsp27, Human.
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Human Lung lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: Hsp27 knockout Hela cell lysate;
    Predicted MW: 23kd
    Observed MW: 23kd
    Primary Ab: 3µg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Porcine Heart lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Hela cell lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

The antibody is a rabbit polyclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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