Polyclonal Antibody to Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) Packages (Simulation)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) Packages (Simulation)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) DAB staining on IHC-P; Samples: Rat Pancreas Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat NSE Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) DAB staining on IHC-P;
    Samples: Rat Cerebrum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) DAB staining on IHC-P;
    Samples: Rat Testis Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA537Ra01.jpg Figure. Western Blot; Sample: Recombinant NSE, Rat.
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Hela cell lysate
    Primary Ab: 1ug/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: NSE knockout HepG2 cell lysate;
    Predicted MW: 47kd
    Observed MW: 52kd
    Primary Ab: 1µg/ml Rabbit Anti-Rat NSE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase, Neuron Specific (NSE) Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-Mouse NSE Polyclonal Antibody (Catalog PAA537Ra01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

The antibody is a rabbit polyclonal antibody raised against NSE. It has been selected for its ability to recognize NSE in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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