ELISA Kit for Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • ELISA Kit for Enolase, Neuron Specific (NSE) Packages (Simulation)
  • ELISA Kit for Enolase, Neuron Specific (NSE) Packages (Simulation)
  • ELISA Kit for Enolase, Neuron Specific (NSE) Results demonstration
  • SEA537Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Enolase, Neuron Specific (NSE)

This assay has high sensitivity and excellent specificity for detection of Enolase, Neuron Specific (NSE).
No significant cross-reactivity or interference between Enolase, Neuron Specific (NSE) and analogues was observed.

Recovery of the ELISA Kit for Enolase, Neuron Specific (NSE)

Matrices listed below were spiked with certain level of recombinant Enolase, Neuron Specific (NSE) and the recovery rates were calculated by comparing the measured value to the expected amount of Enolase, Neuron Specific (NSE) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 95-103 99
heparin plasma(n=5) 78-93 87

Precision of the ELISA Kit for Enolase, Neuron Specific (NSE)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase, Neuron Specific (NSE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase, Neuron Specific (NSE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Enolase, Neuron Specific (NSE)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Enolase, Neuron Specific (NSE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 78-101% 82-95% 88-95%
EDTA plasma(n=5) 95-103% 98-105% 80-93% 78-92%
heparin plasma(n=5) 91-99% 81-102% 97-105% 93-105%

Stability of the ELISA Kit for Enolase, Neuron Specific (NSE)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Enolase, Neuron Specific (NSE)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Enolase, Neuron Specific (NSE)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enolase, Neuron Specific (NSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enolase, Neuron Specific (NSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enolase, Neuron Specific (NSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enolase, Neuron Specific (NSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Rattus norvegicus (Rat) Applications (RESEARCH USE ONLY!)
RPA537Ra01 Recombinant Enolase, Neuron Specific (NSE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA537Ra01 Polyclonal Antibody to Enolase, Neuron Specific (NSE) WB,IHC
FAA537Ra01 Anti-Enolase, Neuron Specific (NSE) Polyclonal Antibody Flow cytometry.
LAA537Ra81 FITC-Linked Polyclonal Antibody to Enolase, Neuron Specific (NSE) WB; IHC; ICC; IF.
LAA537Ra71 Biotin-Linked Polyclonal Antibody to Enolase, Neuron Specific (NSE) WB; IHC; ICC.
MAA537Ra21 Monoclonal Antibody to Enolase, Neuron Specific (NSE) WB; IHC; ICC; IP.
SEA537Ra ELISA Kit for Enolase, Neuron Specific (NSE) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA537Ra Mini Samples ELISA Kit for Enolase, Neuron Specific (NSE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA537Ra Multiplex Assay Kit for Enolase, Neuron Specific (NSE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK