Active Programmed Cell Death Protein 1 Ligand 1 (PDL1)

CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1

  • Active Programmed Cell Death Protein 1 Ligand 1 (PDL1) Packages (Simulation)
  • Active Programmed Cell Death Protein 1 Ligand 1 (PDL1) Packages (Simulation)
  • APA788Hu61.png Figure. SDS-PAGE
  • Active Programmed Cell Death Protein 1 Ligand 1 (PDL1) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Besides, Programmed Cell Death Protein 1 (PDCD1) has been identified as an interactor of PDCD1LG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDCD1LG1 and recombinant human PDCD1. Briefly, PDCD1LG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDCD1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PDCD1LG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PDCD1LG1 and PDCD1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of PDCD1LG1 with PDCD1.

USAGE of the Active Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Programmed Cell Death Protein 1 Ligand 1 (PDL1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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