Active Insulin Like Growth Factor 1 (IGF1)
IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
- Product No.APA050Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point7.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors!US$ 700
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For more details, please contact local distributors! US$ 10500
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- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. SDS-PAGE; Sample: Active recombinant IGF1, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Insulin Like Growth Factor 1 (IGF1)
Insulin-like growth factor I (IGF1), is a hormone similar in molecular structure to insulin but have a much higher growth-promoting activity, it belongs to a family of proteins involved in mediating growth and development. It is reported that IGF1 induces the proliferation, migration, differentiation of a large types of cells including the MCF-7 breast cancer cell line. To test the effect of growth factors on proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IGF-1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of IGF1 was shown in Figure2. It was obvious that IGF1 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is typically 8.66~17.19 ng/mL.
USAGE of the Active Insulin Like Growth Factor 1 (IGF1)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Insulin Like Growth Factor 1 (IGF1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Insulin Like Growth Factor 1 (IGF1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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