Active Insulin Like Growth Factor 1 (IGF1)

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

  • Active Insulin Like Growth Factor 1 (IGF1) Packages (Simulation)
  • Active Insulin Like Growth Factor 1 (IGF1) Packages (Simulation)
  • Active Insulin Like Growth Factor 1 (IGF1) Figure. Gene Sequencing (Extract)
  • APA050Hu01.png Figure. SDS-PAGE
  • Active Insulin Like Growth Factor 1 (IGF1) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Insulin Like Growth Factor 1 (IGF1)

Figure. The binding activity of IGF1 with IGFBP4.
Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. IGF1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF1 has a molecular weight of 7,649 Daltons. Besides, Insulin Like Growth Factor Binding Protein 4 (IGFBP4) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGF1 and recombinant human IGFBP4. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to IGFBP4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IGF1 and IGFBP4 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Insulin Like Growth Factor 1 (IGF1)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Insulin Like Growth Factor 1 (IGF1)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Insulin Like Growth Factor 1 (IGF1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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