Polyclonal Antibody to Histone H1 (H1)
- Product No.UPAC527Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download n/a
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human H1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Rat Spleen lysate; Lane2: Mouse Spleen lysate; Lane3: Hela cell lysate; Lane4: Jurkat cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Human H1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Histone H1 (H1)
The antibody is a rabbit polyclonal antibody raised against H1. It has been selected for its ability to recognize H1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Histone H1 (H1)
Western blotting: 0.1-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Histone H1 (H1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Histone H1 (H1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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