Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

  • Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) DAB staining on IHC-P;
    Sample: Mouse Cerebrum Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat HSPA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) DAB staining on IHC-P;
    Sample: Mouse Cerebellum Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat HSPA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) DAB staining on IHC-P;
    Sample: Mouse Testis Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat HSPA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC343Ra01.jpg Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Testis lysate; Lane3: A549 cell lysate; Primary Ab: 0.1µg/ml Rabbit Anti-Rat HSPA5 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

The antibody is a rabbit polyclonal antibody raised against HSPA5. It has been selected for its ability to recognize HSPA5 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

Western blotting: 0.5-2µg/mL;1:130-520
Immunohistochemistry: 5-20µg/mL;1:13-52
Immunocytochemistry: 5-20µg/mL;1:13-52
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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