Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein
- Product No.UPAA822Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA822Hu02-Recombinant Heat Shock Protein 60 (Hsp60)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
-
FOB
US$ 47
For more details, please contact local distributors!US$ 111
For more details, please contact local distributors! US$ 158
For more details, please contact local distributors! US$ 395
For more details, please contact local distributors! US$ 1580
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
-
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.01µg/ml Rabbit Anti-Human Hsp60 Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: A549 cell lysate;
Primary Ab: 0.01µg/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # PAA822Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
-
Human Hela cell was stained with 20µg/ml rabbit Anti-human Hsp60 Polyclonal Antibody (Catalog PAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
-
ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
The antibody is a rabbit polyclonal antibody raised against Hsp60. It has been selected for its ability to recognize Hsp60 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service