Polyclonal Antibody to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)
EMA; GA2; MADD; Glutaric Aciduria II
- Product No.PAE527Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPE527Mu01-Recombinant Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 108
For more details, please contact local distributors!US$ 252
For more details, please contact local distributors! US$ 360
For more details, please contact local distributors! US$ 900
For more details, please contact local distributors! US$ 3600
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse ETFa Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse ETFa Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse ETFa Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse ETFa Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Mouse ETFa Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse ETFa Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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WB Image
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Western Blot; Sample: Lane1: Mouse Lung lysate; Lane2: Mouse Heart lysate; Lane3: Mouse Cerebrum lysate
Primary Ab: 3µg/mL Rabbit Anti-Mouse ETFa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Mouse Liver lysate; Lane3: Human HepG2 cell lysate
Primary Ab: 0.25µg/mL Rabbit Anti-Mouse ETFa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)
The antibody is a rabbit polyclonal antibody raised against ETFa. It has been selected for its ability to recognize ETFa in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPE527Mu01 | Recombinant Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE527Mu01 | Polyclonal Antibody to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) | WB; IHC; ICC; IP. |