Polyclonal Antibody to Cytokeratin 18 (CK18)
KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
- Product No.PAB231Hu03
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB231Hu03-Recombinant Cytokeratin 18 (CK18)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.27mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 236
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For more details, please contact local distributors! US$ 3370
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P;
Sample: Rat Uterus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: Hela cell lysate; Lane3: A431 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: HepG2 cell lysate; Lane4: Hela cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CK18 Polyclonal Antibody (Catalog PAB231Hu03, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Cytokeratin 18 (CK18)
The antibody is a rabbit polyclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Cytokeratin 18 (CK18)
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Cytokeratin 18 (CK18)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Cytokeratin 18 (CK18)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service