Polyclonal Antibody to Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- Product No.PAB340Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB340Hu01-Recombinant Beta Actin (ACTB)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 110
For more details, please contact local distributors!US$ 258
For more details, please contact local distributors! US$ 368
For more details, please contact local distributors! US$ 920
For more details, please contact local distributors! US$ 3680
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human ACTb Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: Human A875 cell lysate; Primary Ab: 3µg/ml Rabbit Anti-Human ACTb Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Human Lung lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human BXPC3 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse RAW264.7 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Lung lysate; Lane2: Hela cell lysate; Lane3: MCF7 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human ACTB Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Beta Actin (ACTB)
The antibody is a rabbit polyclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Beta Actin (ACTB)
Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Beta Actin (ACTB)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Beta Actin (ACTB)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB340Hu01 | Recombinant Beta Actin (ACTB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB340Hu01 | Polyclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| CAB340Hu01 | Anti-Beta Actin (ACTB) Polyclonal Antibody | Loading Control of WB. |
| MAB340Hu22 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu23 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu24 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu21 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu25 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| CAB340Hu22 | Anti-Beta Actin (ACTB) Monoclonal Antibody | Loading Control of WB |