Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)
HL; Hydroxymethylglutaricaciduria; Hydroxymethylglutaryl-CoA lyase, mitochondrial
- Product No.PAD685Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPD685Hu01-Recombinant 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 245
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For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
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WB Image
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Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Cerebrum lysate; Lane4: Porcine Heart lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Cerebrum lysate; Lane7: A431 cell lysate
Primary Ab: 2μg/ml Rabbit Anti-Human HMGCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)
The antibody is a rabbit polyclonal antibody raised against HMGCL. It has been selected for its ability to recognize HMGCL in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPD685Hu01 | Recombinant 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD685Hu01 | Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL) | WB; IHC; ICC; IP. |