Mouse Model for Acute Lung Injury (ALI)

Acute Respiratory Distress Syndrome

  • Mouse Model for Acute Lung Injury (ALI) Packages (Simulation)
  • Mouse Model for Acute Lung Injury (ALI) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Model Evaluation of the Mouse Model for Acute Lung Injury (ALI)

1.Observation of lung injury:
Lung injury can be seen with the naked eye, large area of bleeding and exudation, can directly reflect the degree of lung injury.
2.Lung wet/dry weight ratio:
Lung wet/dry weight ratio is a direct indicator of pulmonary edema, and it is also a sensitive indicator of the severity of lung injury. After the occurrence acute lung injury (ALI), a large amount of fluid to the alveolar and interstitial fluid, leading to increased weight of the lungs, while the dry weight of the lung is not affected.
After killing the rats, cut trachea from whole lung and wet weight, dry the lung on 65℃ for 24hrs, and then calculate lung wet/ dry weight ratio.

Pathological Results of the Mouse Model for Acute Lung Injury (ALI)

Take the left lung 4% poly formaldehyde fixed, and then the normal lung tissue dehydration, paraffin embedded, sliced (thickness of 5μm), HE staining.
HE staining of lung tissue can directly response the degree of lung injury, ALI tissue pathology mainly in alveolar neutrophils and red blood cells exudation, alveolar wall transparent membrane formation. HE staining after can be in inflammatory cells was observed under low magnification infiltration, edema and injury.
At high magnification, the alveolar structure can be identified, and the degree of lung injury can be assessed and evaluated.
Scoring method: take 6 field of vision for bleeding and edema score.
0 points: no damage
1 points: <25% damage area
2 points: 25%~50% damage area
3 points: 50%~75% damage area
4 points: >75% damage area

Cytokines Level of the Mouse Model for Acute Lung Injury (ALI)

Anatomy of left lobectomy trachea, bronchial intubation, precooled sterile normal saline 1 ml, bronchoalveolar lavage 3 times, recycling bronchial alveolar lavage fluid (BALF) to a sterile centrifuge tube, 1500 R/min, 10 mins, collected and stored on 80℃, using ELISA kit for assay of TNF-a, IL-6, IL-1 beta and other cytokins in BALF.

Statistical Analysis of the Mouse Model for Acute Lung Injury (ALI)

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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