Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
DnaK; 70 Kilodalton Heat Shock Proteins
- Product No.UMAA873Hu24
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C4
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies. - Download n/a
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: K562 cell lysate; Lane3: A431 cell lysate;
Primary Ab: 0.2µg/ml Mouse Anti-Human HSP70 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
The antibody is a mouse monoclonal antibody raised against HSP70. It has been selected for its ability to recognize HSP70 in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
Western blotting: 0.1-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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