Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1)

DCDC3A; DCLK; DCAMKL1; Doublecortin And CaM Kinase-Like 1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1

  • Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1) Packages (Simulation)
  • Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1) Packages (Simulation)
  • Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1) DAB staining on IHC-P; Sample: Human Cerebellum Tissue; Primary Ab: 30µg/ml Mouse Anti-Human DCLK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • MAF390Hu23.jpg Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2μg/ml Mouse Anti-Human DCLK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1) Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human DCLK1 Monoclonal Antibody (Catalog MAF390Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1)

The antibody is a mouse monoclonal antibody raised against DCLK1. It has been selected for its ability to recognize DCLK1 in immunohistochemical staining and western blotting.

USAGE of the Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1)

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Monoclonal Antibody to Doublecortin Like Kinase 1 (DCLK1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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