Monoclonal Antibody to Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- Product No.MAB340Mi21
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C7
- Ig Isotype IgG2b Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB340Mi01-Recombinant Beta Actin (ACTB)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreOryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 128
For more details, please contact local distributors!US$ 300
For more details, please contact local distributors! US$ 428
For more details, please contact local distributors! US$ 1070
For more details, please contact local distributors! US$ 4280
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10µg/ml Mouse Anti-Multi-species ACTb Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Amygdalitis Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue;
Primary Ab: 20µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Skeletal muscle Tissue;
Primary Ab: 20µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Heart Tissue
Primary Ab: 20µg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Skeletal muscle Tissue
Primary Ab: 20µg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Tonsil Tissue
Primary Ab: 10µg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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WB Image
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Rat Liver lysate; Lane4: Rat Kidney lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Kidney lysate; Lane7: Mouse Liver lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Mouse Kidney lysate; Lane2: Rabbit Liver lysate; Lane3: Bovine Liver lysate; Lane4: Bovine Kidney lysate; Lane5: Canine Liver lysate; Lane6: Canine Kidney lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Mouse Cerebrum lysate; Lane4: Mouse Liver lysate; Lane5: Rat Cerebrum lysate; Lane6: Rat Liver lysate; Lane7: Cavia Cerebrum lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Porcine Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Gallus Cerebrum lysate; Lane5: Equine Liver lysate; Lane6: Caprine Cerebrum lysate;
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: HL60 cell lysate; Lane3: THP1 cell lysate; Lane4: OS-RC-2 cell lysate; Lane5: HEC-1B cell lysate; Lane6: MDA-MB-435S cell lysate; Lane7: T-47D cell lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Beta Actin (ACTB)
The antibody is a mouse monoclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Beta Actin (ACTB)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Beta Actin (ACTB)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Beta Actin (ACTB)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus) | Applications (RESEARCH USE ONLY!) |
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| SCB340Mi | CLIA Kit for Beta Actin (ACTB) | Chemiluminescent immunoassay for Antigen Detection. |
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| KSB340Mi01 | ELISA Kit DIY Materials for Beta Actin (ACTB) | Main materials for "Do It (ELISA Kit) Yourself". |
| PGB340Mi01 | Primer Pair for Beta Actin (ACTB) | PCR |