Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
KLa; Klotho Alpha
(Note: Up to 8-plex in one testing reaction)
- Product No.ULMH757Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range1.95-2000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.65 pg/mL.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Download Instruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
This assay has high sensitivity and excellent specificity for detection of Klotho (KL).
No significant cross-reactivity or interference between Klotho (KL) and analogues was observed.
Recovery of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
Matrices listed below were spiked with certain level of recombinant Klotho (KL) and the recovery rates were calculated by comparing the measured value to the expected amount of Klotho (KL) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-101 | 92 |
| EDTA plasma(n=5) | 78-96 | 80 |
| heparin plasma(n=5) | 84-98 | 89 |
| sodium citrate plasma(n=5) | 80-93 | 89 |
Precision of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Klotho (KL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-102% | 78-91% | 89-97% | 79-90% |
| EDTA plasma(n=5) | 80-96% | 86-101% | 89-104% | 80-93% |
| heparin plasma(n=5) | 92-105% | 87-95% | 97-105% | 80-89% |
| sodium citrate plasma(n=5) | 78-102% | 97-105% | 80-90% | 79-94% |
Stability of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle of the Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay)
Anti-Klotho (KL) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Klotho (KL) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Klotho (KL) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Klotho (KL) in the sample and corresponding value of MFI (Median Fluorescence Intensity).
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