Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

F1; B-50; PP46; Neuron Growth-Associated Protein 43; Neuromodulin; Axonal Membrane Protein GAP-43; Protein F1; Calmodulin-Binding Protein P-57; Neural Phosphoprotein B-50

(Note: Up to 8-plex in one testing reaction)

  • Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

This assay has high sensitivity and excellent specificity for detection of Growth Associated Protein 43 (GAP43).
No significant cross-reactivity or interference between Growth Associated Protein 43 (GAP43) and analogues was observed.

Recovery of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

Matrices listed below were spiked with certain level of recombinant Growth Associated Protein 43 (GAP43) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Associated Protein 43 (GAP43) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-89 85
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 92-103 97

Precision of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Associated Protein 43 (GAP43) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Associated Protein 43 (GAP43) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Associated Protein 43 (GAP43) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 90-103% 92-99% 93-101%
EDTA plasma(n=5) 93-101% 86-101% 94-103% 85-99%
heparin plasma(n=5) 98-105% 81-98% 97-105% 87-95%

Stability of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Test principle of the Multiplex Assay Kit for Growth Associated Protein 43 (GAP43) ,etc. by FLIA (Flow Luminescence Immunoassay)

Anti-Growth Associated Protein 43 (GAP43) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Growth Associated Protein 43 (GAP43) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Growth Associated Protein 43 (GAP43) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Growth Associated Protein 43 (GAP43) in the sample and corresponding value of MFI (Median Fluorescence Intensity).

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