Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
MTLRP; Growth Hormone-Releasing Peptide
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA991Ov
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range9.77-10000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.257 pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Download Instruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
-
FOB
US$ 505
For more details, please contact local distributors! US$ 525
For more details, please contact local distributors! US$ 554
For more details, please contact local distributors! US$ 593
For more details, please contact local distributors! US$ 632
For more details, please contact local distributors! US$ 690
For more details, please contact local distributors! US$ 778
For more details, please contact local distributors!US$ 972
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
Matrices listed below were spiked with certain level of recombinant Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-102 | 91 |
| EDTA plasma(n=5) | 85-99 | 92 |
| heparin plasma(n=5) | 95-103 | 99 |
| sodium citrate plasma(n=5) | 81-101 | 90 |
Precision of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-93% | 89-98% | 98-105% | 89-97% |
| EDTA plasma(n=5) | 86-97% | 90-104% | 84-95% | 91-98% |
| heparin plasma(n=5) | 78-104% | 79-89% | 95-105% | 80-99% |
| sodium citrate plasma(n=5) | 94-101% | 79-97% | 80-102% | 93-104% |
Stability of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle of the Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Ovis aries; Ovine (Sheep) | Applications (RESEARCH USE ONLY!) |
| CEA991Ov | ELISA Kit for Ghrelin (GHRL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA991Ov | Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |