Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

CEBPZ; CHOP; CHOP10; GADD153; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible GADD153

(Note: Up to 8-plex in one testing reaction)

  • Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

This assay has high sensitivity and excellent specificity for detection of DNA Damage Inducible Transcript 3 (DDIT3).
No significant cross-reactivity or interference between DNA Damage Inducible Transcript 3 (DDIT3) and analogues was observed.

Recovery of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

Matrices listed below were spiked with certain level of recombinant DNA Damage Inducible Transcript 3 (DDIT3) and the recovery rates were calculated by comparing the measured value to the expected amount of DNA Damage Inducible Transcript 3 (DDIT3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-94 89
EDTA plasma(n=5) 92-101 97
heparin plasma(n=5) 85-99 91
sodium citrate plasma(n=5) 84-105 97

Precision of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNA Damage Inducible Transcript 3 (DDIT3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNA Damage Inducible Transcript 3 (DDIT3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DNA Damage Inducible Transcript 3 (DDIT3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 82-94% 94-104% 95-105%
EDTA plasma(n=5) 99-105% 79-90% 90-98% 88-98%
heparin plasma(n=5) 80-102% 83-105% 96-105% 97-105%
sodium citrate plasma(n=5) 87-103% 86-95% 88-101% 78-95%

Stability of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Test principle of the Multiplex Assay Kit for DNA Damage Inducible Transcript 3 (DDIT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

Anti-DNA Damage Inducible Transcript 3 (DDIT3) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which DNA Damage Inducible Transcript 3 (DDIT3) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-DNA Damage Inducible Transcript 3 (DDIT3) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of DNA Damage Inducible Transcript 3 (DDIT3) in the sample and corresponding value of MFI (Median Fluorescence Intensity).

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