Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

5HT; Serotonin; N-Methyl-Gamma

(Note: Up to 8-plex in one testing reaction)

  • Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine (5-HT).
No significant cross-reactivity or interference between 5-Hydroxytryptamine (5-HT) and analogues was observed.

Recovery of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

Matrices listed below were spiked with certain level of 5-Hydroxytryptamine (5-HT) and the recovery rates were calculated by comparing the measured value to the expected amount of 5-Hydroxytryptamine (5-HT) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 86
EDTA plasma(n=5) 81-101 92
heparin plasma(n=5) 78-90 85
sodium citrate plasma(n=5) 88-102 92

Precision of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine (5-HT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine (5-HT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-Hydroxytryptamine (5-HT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-97% 94-103% 88-101% 94-102%
EDTA plasma(n=5) 82-97% 97-105% 92-101% 89-96%
heparin plasma(n=5) 79-99% 94-101% 87-101% 92-101%
sodium citrate plasma(n=5) 84-97% 88-98% 85-95% 83-96%

Stability of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Test principle of the Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay)

Anti-5-Hydroxytryptamine (5-HT) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptamine (5-HT) and unlabeled 5-Hydroxytryptamine (5-HT) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptamine (5-HT). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample, the intensity of MFI developed is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample.

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