High Sensitive ELISA Kit for Nitrotyrosine (NT)
3-Nitro-L-Tyrosine; 3-Nitrotyrosine
- Product No.HEB863Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range1.23-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.52ng/mL.
- Sample Typeserum, plasma and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 622
For more details, please contact local distributors!US$ 889
For more details, please contact local distributors! US$ 4001
For more details, please contact local distributors! US$ 7557
For more details, please contact local distributors! US$ 62230
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Nitrotyrosine (NT).
No significant cross-reactivity or interference between High Sensitive Nitrotyrosine (NT) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
Matrices listed below were spiked with certain level of High Sensitive Nitrotyrosine (NT) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Nitrotyrosine (NT) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-104 | 94 |
| EDTA plasma(n=5) | 84-99 | 96 |
| heparin plasma(n=5) | 98-105 | 102 |
Precision of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Nitrotyrosine (NT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Nitrotyrosine (NT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Nitrotyrosine (NT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-96% | 90-102% | 84-97% | 85-101% |
| EDTA plasma(n=5) | 92-101% | 87-101% | 78-95% | 89-97% |
| heparin plasma(n=5) | 78-101% | 93-101% | 79-92% | 93-102% |
Stability of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the High Sensitive ELISA Kit for Nitrotyrosine (NT)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Nitrotyrosine (NT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Nitrotyrosine (NT) and unlabeled High Sensitive Nitrotyrosine (NT) (Standards or samples) with the pre-coated antibody specific to High Sensitive Nitrotyrosine (NT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Nitrotyrosine (NT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Nitrotyrosine (NT) in the sample.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Pan-species (General) | Applications (RESEARCH USE ONLY!) |
| CPB863Ge11 | BSA Conjugated Nitrotyrosine (NT) | Immunogen; SDS-PAGE; WB. |
| CPB863Ge21 | OVA Conjugated Nitrotyrosine (NT) | Immunogen; SDS-PAGE; WB. |
| PAB863Ge01 | Polyclonal Antibody to Nitrotyrosine (NT) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEB863Ge | ELISA Kit for Nitrotyrosine (NT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB863Ge | High Sensitive ELISA Kit for Nitrotyrosine (NT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB863Ge | Multiplex Assay Kit for Nitrotyrosine (NT) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB863Ge11 | ELISA Kit DIY Materials for Nitrotyrosine (NT) | Main materials for "Do It (ELISA Kit) Yourself". |