High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

NT-Pro-BNP; ; N-BNP

  • High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Packages (Simulation)
  • High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Packages (Simulation)
  • High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Results demonstration
  • HEA485Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

This assay has high sensitivity and excellent specificity for detection of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP).
No significant cross-reactivity or interference between High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and analogues was observed.

Recovery of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

Matrices listed below were spiked with certain level of recombinant High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-101 89
EDTA plasma(n=5) 92-101 95
heparin plasma(n=5) 86-93 89

Precision of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 84-98% 86-93% 96-104%
EDTA plasma(n=5) 81-101% 96-103% 84-95% 98-105%
heparin plasma(n=5) 85-95% 95-105% 99-105% 87-94%

Stability of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and unlabeled High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) (Standards or samples) with the pre-coated antibody specific to High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample.

Related products

Catalog No. Organism species: Rattus norvegicus (Rat) Applications (RESEARCH USE ONLY!)
RPA485Ra01 Recombinant N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Positive Control; Immunogen; SDS-PAGE; WB.
CPA485Ra21 OVA Conjugated N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Immunogen; SDS-PAGE; WB.
CPA485Ra22 OVA Conjugated N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Immunogen; SDS-PAGE; WB.
CPA485Ra31 KLH Conjugated N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Immunogen; SDS-PAGE; WB.
PAA485Ra01 Polyclonal Antibody to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) WB; IHC; ICC; IP.
PAA485Ra08 Polyclonal Antibody to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) WB; IHC; ICC; IP.
LAA485Ra79 Biotin-Linked Polyclonal Antibody to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) WB; IHC; ICC.
MAA485Ra28 Monoclonal Antibody to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) WB; IHC; ICC; IP.
SEA485Ra ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA485Ra ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA485Ra High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA485Ra Multiplex Assay Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK