High Sensitive ELISA Kit for Immunoglobulin G (IgG)
- Product No.UHEA544Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.5ng/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Immunoglobulin G (IgG).
No significant cross-reactivity or interference between High Sensitive Immunoglobulin G (IgG) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
Matrices listed below were spiked with certain level of recombinant High Sensitive Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Immunoglobulin G (IgG) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-99 | 93 |
| EDTA plasma(n=5) | 84-101 | 91 |
| heparin plasma(n=5) | 87-102 | 96 |
Precision of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-104% | 81-101% | 87-94% | 99-105% |
| EDTA plasma(n=5) | 78-104% | 81-105% | 90-98% | 93-102% |
| heparin plasma(n=5) | 85-103% | 80-101% | 80-104% | 90-97% |
Stability of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the High Sensitive ELISA Kit for Immunoglobulin G (IgG)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Immunoglobulin G (IgG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Immunoglobulin G (IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Immunoglobulin G (IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Immunoglobulin G (IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.