Eukaryotic Interleukin 23 (IL23)
- Product No.UEPX262Mu62
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourceEukaryotic expression
- Host293F
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Predicted Molecular Mass58.81kDa
- Accurate Molecular Mass75kDa(Analysis of differences refer to the manual)
- Residues & TagsMet23~Ser335+IGSGSSRGGSGSGGSGGGGSK+Leu20~Ala196 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.5
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - Download n/a
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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For more details, please contact local distributors!US$ 328
For more details, please contact local distributors! US$ 655
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For more details, please contact local distributors! US$ 4913
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SEQUENCE of the Eukaryotic Interleukin 23 (IL23)
Interleukin-23 (IL-23) is a heterodimeric pro-inflammatory cytokine composed of two subunits: p19 (unique to IL-23) and p40 (shared with IL-12). Primarily produced by activated dendritic cells, macrophages, and other antigen-presenting cells, IL-23 plays a pivotal role in immune responses, particularly in promoting the differentiation and maintenance of T helper 17 (Th17) cells. Th17 cells secrete IL-17, IL-22, and other inflammatory mediators, which are crucial for host defense against extracellular pathogens but also contribute to autoimmune and chronic inflammatory diseases, such as psoriasis, inflammatory bowel disease (IBD), and rheumatoid arthritis.Besides,Janus Kinase 2 (JAK2) has been identified as an interactor of IL-23, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IL-23 and recombinant rat JAK2. Briefly, IL-23 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL-23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse IL-23 and recombinant rat JAK2 was shown in Figure 1, the EC50 for this effect is 0.157µg/mL.
USAGE of the Eukaryotic Interleukin 23 (IL23)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Eukaryotic Interleukin 23 (IL23)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Eukaryotic Interleukin 23 (IL23)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Immunoprecipitation (IP) Experiment Service
TBST Buffer
TBS Buffer
TRIS Buffer
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| UAPX262Mu62 | Active Interleukin 23 (IL23) | Cell culture; Activity Assays. |
| UAPX262Mu61 | Active Interleukin 23 (IL23) | Cell culture; Activity Assays. |
| URPX262Mu01 | Recombinant Interleukin 23 (IL23) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPX262Mu61 | Eukaryotic Interleukin 23 (IL23) | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPX262Mu02 | Recombinant Interleukin 23 (IL23) | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPX262Mu03 | Recombinant Interleukin 23 (IL23) | Positive Control; Immunogen; SDS-PAGE; WB. |
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