ELISA Kit for Protein Zero, Myelin (MPZ)

P0; DSS; CHM; CMT4E; CMT1; CMT1B; CMT2I; CMT2J; CMTDI3; HMSNIB; MPP; Myelin Protein Zero; Charcot-Marie-Tooth Neuropathy 1B; Myelin peripheral protein

  • ELISA Kit for Protein Zero, Myelin (MPZ) Packages (Simulation)
  • ELISA Kit for Protein Zero, Myelin (MPZ) Packages (Simulation)
  • ELISA Kit for Protein Zero, Myelin (MPZ) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Protein Zero, Myelin (MPZ)

This assay has high sensitivity and excellent specificity for detection of Protein Zero, Myelin (MPZ).
No significant cross-reactivity or interference between Protein Zero, Myelin (MPZ) and analogues was observed.

Precision of the ELISA Kit for Protein Zero, Myelin (MPZ)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the ELISA Kit for Protein Zero, Myelin (MPZ)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Protein Zero, Myelin (MPZ)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Protein Zero, Myelin (MPZ)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Protein Zero, Myelin (MPZ) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Protein Zero, Myelin (MPZ) and unlabeled Protein Zero, Myelin (MPZ) (Standards or samples) with the pre-coated antibody specific to Protein Zero, Myelin (MPZ). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Protein Zero, Myelin (MPZ) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Protein Zero, Myelin (MPZ) in the sample.

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