ELISA Kit for C-Type Natriuretic Peptide (CNP)
- Product No.SEA721Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.2pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 574
For more details, please contact local distributors!US$ 820
For more details, please contact local distributors! US$ 3690
For more details, please contact local distributors! US$ 6970
For more details, please contact local distributors! US$ 57400
For more details, please contact local distributors!
Specificity of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
This assay has high sensitivity and excellent specificity for detection of C-Type Natriuretic Peptide (CNP).
No significant cross-reactivity or interference between C-Type Natriuretic Peptide (CNP) and analogues was observed.
Recovery of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
Matrices listed below were spiked with certain level of recombinant C-Type Natriuretic Peptide (CNP) and the recovery rates were calculated by comparing the measured value to the expected amount of C-Type Natriuretic Peptide (CNP) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-92 | 87 |
EDTA plasma(n=5) | 98-105 | 101 |
heparin plasma(n=5) | 92-101 | 98 |
Precision of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Natriuretic Peptide (CNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Natriuretic Peptide (CNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Type Natriuretic Peptide (CNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 97-105% | 78-98% | 92-99% | 92-101% |
EDTA plasma(n=5) | 80-99% | 85-97% | 94-101% | 80-90% |
heparin plasma(n=5) | 96-105% | 96-104% | 88-96% | 86-102% |
Stability of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for C-Type Natriuretic Peptide (CNP)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Natriuretic Peptide (CNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Natriuretic Peptide (CNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Natriuretic Peptide (CNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Natriuretic Peptide (CNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
GIVEAWAYS
INCREMENT SERVICES
Related products
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA721Mu02 | Recombinant C-Type Natriuretic Peptide (CNP) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA721Mu01 | Recombinant C-Type Natriuretic Peptide (CNP) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA721Mu21 | OVA Conjugated C-Type Natriuretic Peptide (CNP) | Immunogen; SDS-PAGE; WB. |
PAA721Mu01 | Polyclonal Antibody to C-Type Natriuretic Peptide (CNP) | WB; IHC; ICC; IP. |
PAA721Mu02 | Polyclonal Antibody to C-Type Natriuretic Peptide (CNP) | WB; IHC; ICC; IP. |
SEA721Mu | ELISA Kit for C-Type Natriuretic Peptide (CNP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA721Mu | ELISA Kit for C-Type Natriuretic Peptide (CNP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA721Mu | Multiplex Assay Kit for C-Type Natriuretic Peptide (CNP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA721Mu01 | ELISA Kit DIY Materials for C-Type Natriuretic Peptide (CNP) | Main materials for "Do It (ELISA Kit) Yourself". |