ELISA Kit for Heparin (Hep)
Unfractionated heparin
- Product No.UCEV639Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.33ng/mL.
- Sample Typeserum, plasma and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 312
For more details, please contact local distributors!US$ 445
For more details, please contact local distributors! US$ 2003
For more details, please contact local distributors! US$ 3783
For more details, please contact local distributors! US$ 31150
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Heparin (Hep)
This assay has high sensitivity and excellent specificity for detection of Heparin (Hep).
No significant cross-reactivity or interference between Heparin (Hep) and analogues was observed.
Recovery of the ELISA Kit for Heparin (Hep)
Matrices listed below were spiked with certain level of recombinant Heparin (Hep) and the recovery rates were calculated by comparing the measured value to the expected amount of Heparin (Hep) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-104 | 90 |
| EDTA plasma(n=5) | 84-91 | 88 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision of the ELISA Kit for Heparin (Hep)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin (Hep) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin (Hep) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the ELISA Kit for Heparin (Hep)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparin (Hep) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 95-102% | 91-98% | 79-98% |
| EDTA plasma(n=5) | 97-105% | 84-104% | 80-98% | 99-105% |
| heparin plasma(n=5) | 82-103% | 80-89% | 79-97% | 90-97% |
Stability of the ELISA Kit for Heparin (Hep)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Heparin (Hep)
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the ELISA Kit for Heparin (Hep)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Heparin (Hep) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Heparin (Hep) and unlabeled Heparin (Hep) (Standards or samples) with the pre-coated antibody specific to Heparin (Hep). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Heparin (Hep) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Heparin (Hep) in the sample.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Pan-species (General) | Applications (RESEARCH USE ONLY!) |
| UCPV639Ge11 | BSA Conjugated Heparin (Hep) | Immunogen; SDS-PAGE; WB. |
| UCPV639Ge21 | OVA Conjugated Heparin (Hep) | Immunogen; SDS-PAGE; WB. |
| UPAV639Ge01 | Polyclonal Antibody to Heparin (Hep) | WB; IHC; ICC; IP. |
| UCEV639Ge | ELISA Kit for Heparin (Hep) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMV639Ge | Multiplex Assay Kit for Heparin (Hep) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| UKSV639Ge11 | ELISA Kit DIY Materials for Heparin (Hep) | Main materials for "Do It (ELISA Kit) Yourself". |