ELISA Kit for Ferroportin (FPN)

SLC40A1; FPN1; HFE4; IREG1; MTP1; SLC11A3; Solute Carrier Family 40,Member 1; Iron-Regulated Transporter

  • ELISA Kit for Ferroportin (FPN) Packages (Simulation)
  • ELISA Kit for Ferroportin (FPN) Packages (Simulation)
  • ELISA Kit for Ferroportin (FPN) Results demonstration
  • SEC489Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Ferroportin (FPN)

This assay has high sensitivity and excellent specificity for detection of Ferroportin (FPN).
No significant cross-reactivity or interference between Ferroportin (FPN) and analogues was observed.

Precision of the ELISA Kit for Ferroportin (FPN)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferroportin (FPN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferroportin (FPN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the ELISA Kit for Ferroportin (FPN)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Ferroportin (FPN)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Ferroportin (FPN)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferroportin (FPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferroportin (FPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferroportin (FPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferroportin (FPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPC489Mu02 Recombinant Ferroportin (FPN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC489Mu01 Recombinant Ferroportin (FPN) Positive Control; Immunogen; SDS-PAGE; WB.
CPC489Mu21 OVA Conjugated Ferroportin (FPN) Immunogen; SDS-PAGE; WB.
PAC489Mu02 Polyclonal Antibody to Ferroportin (FPN) WB; IHC; ICC; IP.
PAC489Mu01 Polyclonal Antibody to Ferroportin (FPN) WB; IHC; ICC; IP.
SEC489Mu ELISA Kit for Ferroportin (FPN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC489Mu CLIA Kit for Ferroportin (FPN) Chemiluminescent immunoassay for Antigen Detection.
LMC489Mu Multiplex Assay Kit for Ferroportin (FPN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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