ELISA Kit for Corticosterone (Cort)

Corticosrone

  • ELISA Kit for Corticosterone (Cort) Packages (Simulation)
  • ELISA Kit for Corticosterone (Cort) Packages (Simulation)
  • ELISA Kit for Corticosterone (Cort) Results demonstration
  • CEA540Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Corticosterone (Cort)

This assay has high sensitivity and excellent specificity for detection of Corticosterone (Cort).
No significant cross-reactivity or interference between Corticosterone (Cort) and analogues was observed.

Recovery of the ELISA Kit for Corticosterone (Cort)

Matrices listed below were spiked with certain level of Corticosterone (Cort) and the recovery rates were calculated by comparing the measured value to the expected amount of Corticosterone (Cort) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-97 88
EDTA plasma(n=5) 79-89 82
heparin plasma(n=5) 98-105 101

Precision of the ELISA Kit for Corticosterone (Cort)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticosterone (Cort) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticosterone (Cort) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Corticosterone (Cort)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticosterone (Cort) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 93-101% 78-91% 81-105%
EDTA plasma(n=5) 99-105% 78-92% 80-89% 79-95%
heparin plasma(n=5) 84-92% 89-98% 88-98% 80-101%

Stability of the ELISA Kit for Corticosterone (Cort)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Corticosterone (Cort)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Corticosterone (Cort)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticosterone (Cort) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corticosterone (Cort) and unlabeled Corticosterone (Cort) (Standards or samples) with the pre-coated antibody specific to Corticosterone (Cort). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticosterone (Cort) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticosterone (Cort) in the sample.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
UCPA540Ge11 BSA Conjugated Corticosterone (Cort) Immunogen; SDS-PAGE; WB.
UCPA540Ge21 OVA Conjugated Corticosterone (Cort) Immunogen; SDS-PAGE; WB.
UCPA540Ge31 KLH Conjugated Corticosterone (Cort) Immunogen; SDS-PAGE; WB.
UPAA540Ge01 Polyclonal Antibody to Corticosterone (Cort) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
UPAA540Ge02 Polyclonal Antibody to Corticosterone (Cort) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
ULAA540Ge71 Biotin-Linked Polyclonal Antibody to Corticosterone (Cort) WB; IHC; ICC.
ULAA540Ge81 FITC-Linked Polyclonal Antibody to Corticosterone (Cort) WB; IHC; ICC; IF.
UMAA540Ge28 Monoclonal Antibody to Corticosterone (Cort) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
UMAA540Ge21 Monoclonal Antibody to Corticosterone (Cort) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
UMAA540Ge22 Monoclonal Antibody to Corticosterone (Cort) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
ULAA540Ge72 Biotin-Linked Monoclonal Antibody to Corticosterone (Cort) WB; IHC; ICC.
UCEA540Ge ELISA Kit for Corticosterone (Cort) Enzyme-linked immunosorbent assay for Antigen Detection.
UWEA540Ge Wide-range ELISA Kit for Corticosterone (Cort) Enzyme-linked immunosorbent assay for Antigen Detection.
UMEA540Ge Mini Samples ELISA Kit for Corticosterone (Cort) Enzyme-linked immunosorbent assay for Antigen Detection.
UCCA540Ge CLIA Kit for Corticosterone (Cort) Chemiluminescent immunoassay for Antigen Detection.
ULMA540Ge Multiplex Assay Kit for Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
UKSA540Ge11 ELISA Kit DIY Materials for Corticosterone (Cort) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK